Extraction and Purification of Organophosphorus hydrolase Enzyme from Soil Microorganism Pseudomonas diminuta
نویسندگان
چکیده
منابع مشابه
Purification and properties of the phosphotriesterase from Pseudomonas diminuta.
The phosphotriesterase produced from the opd cistron of Pseudomonas diminuta was purified 1500-fold to homogeneity using a combination of gel filtration, ion exchange, hydrophobic, and dye matrix chromatographic steps. This is the first organophosphate triesterase or organophosphofluoridate hydrolyzing enzyme to be purified to homogeneity. The enzyme is a monomeric, spherical protein having a m...
متن کاملEvaluation of the organophosphorus hydrolase enzyme activity in creams and investigation of its stability
The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
متن کاملOptimal Conditions for Extraction and Purification of Penicillinase Enzyme
Background and Objective: The present study aimed to evaluate the optimum conditions for extracting and purifying penicillinase enzyme. The enzyme source was Bacillus licheniformis 6346 obtained from the Iranian Research Organization for Science and Technology. Materials and Methods: B. licheniformis was cultured in a medium containing mineral salts, nitrogen compounds, and cultured carbon sou...
متن کاملPurification and Properties of Nucleoside Hydrolase from Pseudomonas jhorescens*
An enzyme which catalyzes the hydrolytic cleavage of various ribonucleosides to free base and ribose components has been obtained from cells of Pseudomonas fluorescens and purified about 300-fold by fractionation with protamine sulfate, ammonium sulfate, alumina Cy, and diethylaminoethyl cellulose. The preparation thus purEed catalyzes the hydrolysis of about 50 moles of uridine per min per mg ...
متن کاملPurification and properties of nucleoside hydrolase from Pseudomonas fluorescens.
An enzyme which catalyzes the hydrolytic cleavage of various ribonucleosides to free base and ribose components has been obtained from cells of Pseudomonas fluorescens and purified about 300-fold by fractionation with protamine sulfate, ammonium sulfate, alumina Cy, and diethylaminoethyl cellulose. The preparation thus purEed catalyzes the hydrolysis of about 50 moles of uridine per min per mg ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Defence Life Science Journal
سال: 2017
ISSN: 2456-0537,2456-379X
DOI: 10.14429/dlsj.2.12272